arraystar human circrna v2.0  (Agilent technologies)

Journal: Molecular Therapy. Nucleic Acids

Article Title: The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysms

doi: 10.1016/j.omtn.2023.08.017

Figure Lengend Snippet: Circular RNAs are deregulated in human abdominal aortic aneurysm (A) Volcano plot depicting downregulated (51, blue) and upregulated (40, red). Circular RNAs (circRNAs) in human elective human abdominal aortic aneurysm (eAAA, n = 11) vs . control (CTRL, n = 6) aorta specimens, as resulted by array experiments. Log2 fold change and -log10 p value are plotted on the x and y axes, respectively. IDs of circRNAs meant for a first round of validation are highlighted. Statistics: unpaired t test; p values <0.05 are considered significant. (B) Pie chart illustrating the proportion of exonic (89.8%), intronic (5.7%), sense overlapping (3.4%), and antisense (1.1%) array-identified differentially expressed circRNAs. Absolute numbers are further indicated for each group. (C) Real-time quantitative PCR (qPCR) validation of hsa_circ_0005660 (c NFIX ), hsa_circ_0003641 (c ATM ), hsa_circ0042103 (c MYOCD ), hsa_circ003218 (c BMPR2 ), hsa_circ0004771 (c NRIP1 ), and hsa_circ0005615 (c NFATC3 ) differential expression in human eAAA (N = 8) and CTRL (N = 4) aortas. 2 –ddCT was calculated by normalizing on RPLPO . Data are represented as mean ± SEM. Statistics: unpaired t test; p values <0.05 are considered significant. NS, not significant; eAAA, elective AAA.

Article Snippet: The resulting labeled cDNA was then purified and 1 μg was fragmented, heated, and subsequently hybridized with an 8 × 15k commercially available array chip displaying 13,617 human circRNAs (Arraystar, no. AS-S-CR-H-V2.0) for 17 h at 65°C in an Agilent Hybridization Oven.

Techniques: Control, Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative Proteomics

Journal: Medicine

Article Title: Correlation between serum circRNA and thyroid micropapillary carcinoma with cervical lymph node metastasis

doi: 10.1097/MD.0000000000023255

Figure Lengend Snippet: Primers for qRT-PCR.

Article Snippet: CircRNA expression profiling and data analysis were carried out using Arraystar Human CircRNA V2.0 (Agilent).

Techniques:

Journal: Medicine

Article Title: Correlation between serum circRNA and thyroid micropapillary carcinoma with cervical lymph node metastasis

doi: 10.1097/MD.0000000000023255

Figure Lengend Snippet: CircRNA microarray expression in serum between PTMC and PTMC (L) patients. (A) The typical results of circRNA microarray. Each bright spot represents a circRNA gene. (B) Normalized data were plotted as a Box plot to determine the overall characteristics of distribution.

Article Snippet: CircRNA expression profiling and data analysis were carried out using Arraystar Human CircRNA V2.0 (Agilent).

Techniques: Microarray, Expressing

Journal: Medicine

Article Title: Correlation between serum circRNA and thyroid micropapillary carcinoma with cervical lymph node metastasis

doi: 10.1097/MD.0000000000023255

Figure Lengend Snippet: Representative up-regulated and down-regulated circRNAs in PTMC (L) patients compared with that in PTMC patients.

Article Snippet: CircRNA expression profiling and data analysis were carried out using Arraystar Human CircRNA V2.0 (Agilent).

Techniques:

Journal: Medicine

Article Title: Correlation between serum circRNA and thyroid micropapillary carcinoma with cervical lymph node metastasis

doi: 10.1097/MD.0000000000023255

Figure Lengend Snippet: GO and KEGG analysis of the parental genes of differentially expressed circRNAs. The parental genes of the differentially expressed circRNAs were identified using bioinformatics tools. (A) Functions of the upregulated circRNAs PTMC (L) patients were enriched by GO analysis. (B) KEGG pathway analysis of the circRNA parental genes.

Article Snippet: CircRNA expression profiling and data analysis were carried out using Arraystar Human CircRNA V2.0 (Agilent).

Techniques:

Journal: Medicine

Article Title: Correlation between serum circRNA and thyroid micropapillary carcinoma with cervical lymph node metastasis

doi: 10.1097/MD.0000000000023255

Figure Lengend Snippet: Highly matched miRNAs for the differentially expressed circRNAs between PTMC and PTMC (L) patients.

Article Snippet: CircRNA expression profiling and data analysis were carried out using Arraystar Human CircRNA V2.0 (Agilent).

Techniques:

Journal: Medicine

Article Title: Correlation between serum circRNA and thyroid micropapillary carcinoma with cervical lymph node metastasis

doi: 10.1097/MD.0000000000023255

Figure Lengend Snippet: CircRNA-miRNA-mRNA network of 10 differentially expressed circRNAs. (A) Predicted miRNA response elements for hsa_circRNA_404686. (B) Putative circRNA-miRNA-mRNA network of hsa_circRNA-404686 (brown node) mRNAs (blue nodes) and their target miRNAs (red nodes) was constructed using bioinformatics tools.

Article Snippet: CircRNA expression profiling and data analysis were carried out using Arraystar Human CircRNA V2.0 (Agilent).

Techniques: Construct

Journal: Medicine

Article Title: Correlation between serum circRNA and thyroid micropapillary carcinoma with cervical lymph node metastasis

doi: 10.1097/MD.0000000000023255

Figure Lengend Snippet: Validation of circRNAs expression via real-time PCR. (A) Expression of 10 differentially expressed circRNAs in dependent 3 pairs of patient samples were analyzed by real-time PCR. (B) Expression of hsa_circRNA_404686 in additional 3 pairs of patient samples were analyzed by real-time PCR. U6 snRNA was used as internal control. Compared with PTMC, ∗∗ P < .01, student t test.

Article Snippet: CircRNA expression profiling and data analysis were carried out using Arraystar Human CircRNA V2.0 (Agilent).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Medicine

Article Title: Correlation between serum circRNA and thyroid micropapillary carcinoma with cervical lymph node metastasis

doi: 10.1097/MD.0000000000023255

Figure Lengend Snippet: Comparison of RT-PCR verification results and microarray results for circRNAs.

Article Snippet: CircRNA expression profiling and data analysis were carried out using Arraystar Human CircRNA V2.0 (Agilent).

Techniques: Microarray